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Isoelectric Focusing Theory Methodology And Application


Author : Pier Giorgio Righetti
language : en
Publisher: Elsevier
Release Date : 2000-04-01


PDF Download Isoelectric Focusing Theory Methodology And Application Books For free written by Pier Giorgio Righetti and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-04-01 with Science categories.


This book deals with theoretical and practical developments of IEF and offers detailed methodology for many of the commonly used procedures, such as IEF in gels. It is intended both as a reference guide and a practical manual.

Immobilized Ph Gradients Theory And Methodology


Author : Pier Giorgio Righetti
language : en
Publisher: Elsevier
Release Date : 1990-02-07


PDF Download Immobilized Ph Gradients Theory And Methodology Books For free written by Pier Giorgio Righetti and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-02-07 with Science categories.


Immobilized pH gradients (IPG) represent the most advanced development of isoelectric focusing (IEF). Originally developed to overcome all the problems of IEF in soluble amphoteric buffers (CA) (such as pH gradient instability, complexation with CA chemicals, unreproducibility of pH gradients, protein precipitation at the pI), it turned out to be an entirely new technique, quite different in principle and operation from conventional IEF. The book is thus meant to bring the reader up to date with this fast developing field. The book is divided into six chapters containing information on: detailed treatment of all the chemistry of the Immobiline chemicals; theory of pH gradient generation (computer simulations, tables with all the possible pH recipes); all analytical aspects of IPGs, including staining techniques, blotting etc.; two-dimensional maps, with a detailed treatise of advantages and limitations; preparative aspects of IPGs, including comparison with other preparative electrophoretic techniques; some examples of applications, including genetic and forensic analysis, blood polymorphism etc. The book is extensive and up-to-date, while also extensively covering the theory. Clearly written, with easily accessible information, the volume is a worthy asset to the Lab. Techniques series.

Handbook Of Isoelectric Focusing And Proteomics


Author : David Edward Garfin
language : en
Publisher: Academic Press
Release Date : 2005


PDF Download Handbook Of Isoelectric Focusing And Proteomics Books For free written by David Edward Garfin and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Medical categories.


Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Electrophoretic And Isoelectric Focusing Techniques In Fisheries Management


Author : Donald H. Whitmore
language : en
Publisher: CRC Press
Release Date : 1990-09-24


PDF Download Electrophoretic And Isoelectric Focusing Techniques In Fisheries Management Books For free written by Donald H. Whitmore and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-09-24 with Science categories.


Probably the most ubiquitous biochemical method used today for examining the genetics of individuals, populations, or phylogenetic relationships between taxa is electrophoresis. This book has been created to offer a viewpoint regarding current electrophoretic separation methodologies of macromolecules and their major applications to fisheries management. The chapters in this book have been selected and organized into three sections to create a carefully blended mixture of methodologies and applications designed to educate the novice, as well as stimulate interest in professional researchers currently using electrophoresis for their work. The first section includes chapters that discuss the principles that explain the genetic basis of multiple molecular forms of proteins, the theory and practice of DNA analyses, and the methodology of electrophoretic separation of these macromolecules; starch gel electrophoresis as the predominant electrophoretic tool for fisheries genetics; and protein isoelectric focusing and DNA analysis. The second section describes a variety of applications for electrophoretic techniques. The third section presents a discussion and results of experiments conducted by Dennis Powers and his associates regarding the physiological significance of multiple forms of enzymes using the fish Fundulus heteroclitus as a model system. The book features a catalog of nearly 100 enzyme staining recipes and covers new areas in electrophoretic work, such as DNA fingerprinting, genetic tags, mitochondrial DNA methodologies, and genomic manipulation of fish stocks. This book will provide a useful reference resource for fisheries biologists at federal, state, and local levels; fisheries researchers at universities; and students pursuing degrees involving research in fish genetics.

Amino Acids Peptides And Proteins


Author : Royal Society of Chemistry
language : en
Publisher: Royal Society of Chemistry
Release Date : 1984-01-01


PDF Download Amino Acids Peptides And Proteins Books For free written by Royal Society of Chemistry and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984-01-01 with Medical categories.


Indispensable reference source for researchers in the pharmaceutical and allied industries, and at the biology/chemistry interface in academia.

Laboratory Techniques In Biochemistry And Molecular Biology


Author : Thomas Spence Work
language : en
Publisher:
Release Date : 1984


PDF Download Laboratory Techniques In Biochemistry And Molecular Biology Books For free written by Thomas Spence Work and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Biochemistry categories.




Practical Capillary Electrophoresis


Author : Robert Weinberger
language : en
Publisher:
Release Date : 2000


PDF Download Practical Capillary Electrophoresis Books For free written by Robert Weinberger and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.


In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Key Features * Illustrated with detailed diagrams of electrophoretic phenomena * Offers step-by-step methods development schemes * Presents techniques for developing quantitative, robust, and precise methods * Includes an extensive troubleshooting guide * Updates and greatly expands on the first edition-more than 50% of the text is new * Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE